Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.

ABSTRACT:

Objective

To investigate structural and quantitative alterations of gut microbiota in an experimental model of small bowel obstruction.

Method

A rat model of small bowel obstruction was established by using a polyvinyl chloride ring surgically placed surrounding the terminal ileum. The alterations of gut microbiota were studied after intestinal obstruction. Intraluminal fecal samples proximal to the obstruction were collected at different time points (24, 48 and 72 hours after obstruction) and analyzed by 16s rDNA high-throughput sequencing technology and quantitative PCR (qPCR) for target bacterial groups. Furthermore, intestinal claudin-1 mRNA expression was examined by real-time polymerase chain reaction analysis, and serum sIgA, IFABP and TFF3 levels were determined by e

SUBMITTER: Mo J 

PROVIDER: S-EPMC8336877 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature