ZFP451-mediated SUMOylation of SATB2 drives embryonic stem cell differentiation.

ABSTRACT: The establishment of cell fates involves alterations of transcription factor repertoires and repurposing of transcription factors by post-translational modifications. In embryonic stem cells (ESCs), the chromatin organizers SATB2 and SATB1 balance pluripotency and differentiation by activating and repressing pluripotency genes, respectively. Here, we show that conditional Satb2 gene inactivation weakens ESC pluripotency, and we identify SUMO2 modification of SATB2 by the E3 ligase ZFP451 as a potential driver of ESC differentiation. Mutations of two SUMO-acceptor lysines of Satb2 (Satb2^{K →R} ) or knockout of Zfp451 impair the ability of ESCs to silence pluripotency genes and activate differentiation-associated genes in response

SUBMITTER: Antonio Urrutia G 

PROVIDER: S-EPMC8336893 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature