Transcriptional programs of neoantigen-specific TIL in anti-PD-1-treated lung cancers.

ABSTRACT: PD-1 blockade unleashes CD8 T cells¹, including those specific for mutation-associated neoantigens (MANA), but factors in the tumour microenvironment can inhibit these T cell responses. Single-cell transcriptomics have revealed global T cell dysfunction programs in tumour-infiltrating lymphocytes (TIL). However, the majority of TIL do not recognize tumour antigens², and little is known about transcriptional programs of MANA-specific TIL. Here, we identify MANA-specific T cell clones using the MANA functional expansion of specific T cells assay³ in neoadjuvant anti-PD-1-treated non-small cell lung cancers (NSCLC). We use their T cell receptors as a 'barcode' to track and analyse their transcriptional programs in the tumour microenvironment using coupled sing

SUBMITTER: Caushi JX 

PROVIDER: S-EPMC8338555 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature