Survivin drives tumor-associated macrophage reprogramming: a novel mechanism with potential impact for obesity.

ABSTRACT:

Purpose

Recent studies point to adipose-derived stem cells (ASCs) as a link between obesity and cancer. We aimed to determine whether survivin, which is highly secreted by ASCs from subjects with obesity, might drive a pro-tumoral phenotype in macrophages.

Methods

The effect of ASC conditioned medium on the macrophage phenotype was assessed by expression studies. Survivin intracellular localization and internalization were examined by subcellular fractionation and immunofluorescence, respectively. Loss- and gain-of-function studies were performed using adenoviral vectors, and gene expression patterns, migration and invasion capacities of cancer cells were examined. Heterotypic cultures of ASCs, macrophages and cancer cells were established to mimic the tumor microenvironment

SUBMITTER: Benaiges E 

PROVIDER: S-EPMC8338861 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature