Implication of the Type III Effector RipS1 in the Cool-Virulence of Ralstonia solanacearum Strain UW551.

ABSTRACT: Members of the Ralstonia solanacearum species complex cause a variety of wilting diseases across a wide range of hosts by colonizing and blocking xylem vessels. Of great concern are race 3 biovar 2 strains of R. solanacearum capable of causing brown rot of potato at cool temperatures, which are select agents in the United States. To gain a better understanding of cool-virulence mechanisms, we generated libraries of transposon mutants in the cool-virulent R. solanacearum strain UW551 and screened 10,000 mutants using our seedling assay for significantly reduced virulence at 20°C. We found several mutants that exhibited reduced virulence at 28 and 20°C and also mutants that were only affected at the cooler temperature. One mutant of the latter chosen for further study ha

SUBMITTER: Schachterle JK 

PROVIDER: S-EPMC8339923 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature