Single-Cell RNA Sequencing Reveals the Immunological Profiles of Renal Allograft Rejection in Mice.

ABSTRACT: Allograft rejection is a common immunological feature in renal transplantation and is associated with reduced graft survival. A mouse renal allograft rejection model was induced and single-cell RNA sequencing (scRNA-seq) data of CD45⁺ leukocytes in kidney allografts on days 7 (D7) and 15 (D15) after operation was analyzed to reveal a full immunological profiling. We identified 20 immune cell types among 10,921 leukocytes. Macrophages and CD8⁺ T cells constituted the main populations on both timepoints. In the process from acute rejection (AR) towards chronic rejection (CR), the proportion of proliferating and naïve CD8⁺ T cells dropped significantly. Both B cells and neutrophils decreased by about 3 folds. On the contrary, the proportion of macrophages and

SUBMITTER: Shen Q 

PROVIDER: S-EPMC8340022 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature