Comparison of dried blood spot and plasma sampling for untargeted metabolomics.

ABSTRACT:

Introduction

Untargeted metabolomics holds significant promise for biomarker detection and development. In resource-limited settings, a dried blood spot (DBS)-based platform would offer significant advantages over plasma-based approaches that require a cold supply chain.

Objectives

The primary goal of this study was to compare the ability of DBS- and plasma-based assays to characterize maternal metabolites. Utility of the two assays was also assessed in the context of a case-control predictive model in pregnant women living with HIV.

Methods

Untargeted metabolomics was performed on archived paired maternal plasma and DBS from n = 79 women enrolled in a large clinical trial.

Results

A total of 984 named biochemicals were detected across both plasma and DBS sampl

SUBMITTER: Tobin NH 

PROVIDER: S-EPMC8340475 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature