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Synthetic hyperacetylation of nucleosomal histones.

ABSTRACT: We report combinations of a DMAP-based catalyst and phenyl acetate with optimal electron density as a new chemical system for high-yield, selective synthetic acetylation of histone lysine residues. The utility of this chemical system as a unique biologic tool is demonstrated by applying it to Xenopus laevis sperm chromatin.

SUBMITTER: Kajino H

PROVIDER: S-EPMC8341002 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Synthetic hyperacetylation of nucleosomal histones.

Kajino Hidetoshi H Nagatani Tomomi T Oi Miku M Kujirai Tomoya T Kurumizaka Hitoshi H Nishiyama Atsuya A Nakanishi Makoto M Yamatsugu Kenzo K Kawashima Shigehiro A SA Kanai Motomu M

RSC chemical biology 20200519 2

We report combinations of a DMAP-based catalyst and phenyl acetate with optimal electron density as a new chemical system for high-yield, selective synthetic acetylation of histone lysine residues. The utility of this chemical system as a unique biologic tool is demonstrated by applying it to <i>Xenopus laevis</i> sperm chromatin. ...[more]

PMID: 34458748

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