Ndr/Lats Kinases Bind Specific Mob-Family Coactivators through a Conserved and Modular Interface.

ABSTRACT: Ndr/Lats kinases bind Mob coactivator proteins to form complexes that are essential and evolutionarily conserved components of "Hippo" signaling pathways, which control cell proliferation and morphogenesis in eukaryotes. All Ndr/Lats kinases have a characteristic N-terminal regulatory (NTR) region that binds a specific Mob cofactor: Lats kinases associate with Mob1 proteins, and Ndr kinases associate with Mob2 proteins. To better understand the significance of the association of Mob protein with Ndr/Lats kinases and selective binding of Ndr and Lats to distinct Mob cofactors, we determined crystal structures of Saccharomyces cerevisiae Cbk1^NTR-Mob2 and Dbf2^NTR-Mob1 and experimentally assessed determinants of Mob cofactor binding and specificity. This allowed a

SUBMITTER: Parker BW 

PROVIDER: S-EPMC8341033 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature