Chromogranin A regulates gut permeability via the antagonistic actions of its proteolytic peptides.

ABSTRACT:

Aim

A "leaky" gut barrier has been implicated in the initiation and progression of a multitude of diseases, for example, inflammatory bowel disease (IBD), irritable bowel syndrome and celiac disease. Here we show how pro-hormone Chromogranin A (CgA), produced by the enteroendocrine cells, and Catestatin (CST: hCgA_352-372 ), the most abundant CgA-derived proteolytic peptide, affect the gut barrier.

Methods

Colon tissues from region-specific CST-knockout (CST-KO) mice, CgA-knockout (CgA-KO) and WT mice were analysed by immunohistochemistry, western blot, ultrastructural and flowcytometry studies. FITC-dextran assays were used to measure intestinal barrier function. Mice were supplemented with CST or CgA fragment pancreastatin (PST: CgA_250-301 ). The micro

SUBMITTER: Muntjewerff EM 

PROVIDER: S-EPMC8341099 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature