Identifying cysteine residues susceptible to oxidation by photoactivatable atomic oxygen precursors using a proteome-wide analysis.

ABSTRACT: The reactivity profile of atomic oxygen [O(³P)] in the condensed phase has shown a preference for the thiol group of cysteines. In this work, water-soluble O(³P)-precursors were synthesized by adding aromatic burdens and water-soluble sulphonic acid groups to the core structure of dibenzothiophene-S-oxide (DBTO) to study O(³P) reactivity in cell lysates and live cells. The photodeoxygenation of these compounds was investigated using common intermediates, which revealed that an increase in aromatic burdens to the DBTO core structure decreases the total oxidation yield due to competitive photodeoxygenation mechanisms. These derivatives were then tested in cell lysates and live cells to profile changes in cysteine reactivity using the isoTOP-ABPP chemop

SUBMITTER: Isor A 

PROVIDER: S-EPMC8341131 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature