Discovery of Protein Modifications Using Differential Tandem Mass Spectrometry Proteomics.

ABSTRACT: Recent studies have revealed diverse amino acid, post-translational, and noncanonical modifications of proteins in diverse organisms and tissues. However, their unbiased detection and analysis remain hindered by technical limitations. Here, we present a spectral alignment method for the identification of protein modifications using high-resolution mass spectrometry proteomics. Termed SAMPEI for spectral alignment-based modified peptide identification, this open-source algorithm is designed for the discovery of functional protein and peptide signaling modifications, without prior knowledge of their identities. Using synthetic standards and controlled chemical labeling experiments, we demonstrate its high specificity and sensitivity for the discovery of substoichiometric protein modification

SUBMITTER: Cifani P 

PROVIDER: S-EPMC8341206 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature