Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.

ABSTRACT: There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited. Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production. However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals. Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed. Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-spec

SUBMITTER: Margolin E 

PROVIDER: S-EPMC8341435 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature