A self-labeling protein based on the small ultra-red fluorescent protein, smURFP.

ABSTRACT: Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.

SUBMITTER: Machado JH 

PROVIDER: S-EPMC8341759 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature