One-pot fluorescent assay for sensitive detection of APOBEC3A activity.
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ABSTRACT:
SUBMITTER: Wang B
PROVIDER: S-EPMC8341802 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Publications
One-pot fluorescent assay for sensitive detection of APOBEC3A activity.
Wang Bingyao B Zhang Xiong X Wang Yafen Y Chen Kun K Wang Fang F Weng Xiaocheng X Zhou Xiang X
RSC chemical biology 20210430 4
We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors. ...[more]
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