Harnessing the PD-L1 interface peptide for positron emission tomography imaging of the PD-1 immune checkpoint.

ABSTRACT: Interface peptides that mediate protein-protein interactions (PPI) are a class of important lead compounds for designing PPI inhibitors. However, their potential as precursors for radiotracers has never been exploited. Here we report that the interface peptides from programmed death-ligand 1 (PD-L1) can be used in positron emission tomography (PET) imaging of programmed cell death 1 (PD-1) with high accuracy and sensitivity. Moreover, the performance differentiation between murine PD-L1 derived interface peptide (mPep-1) and human PD-L1 derived interface peptide (hPep-1) as PET tracers for PD-1 unveiled an unprecedented role of a non-critical residue in target binding, highlighting the significance of PET imaging as a companion diagnostic in drug development. Collectively, this study not o

SUBMITTER: Hu K 

PROVIDER: S-EPMC8341843 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature