Elongation rate and average length of amyloid fibrils in solution using isotope-labelled small-angle neutron scattering.

ABSTRACT: We demonstrate a solution method that allows both elongation rate and average fibril length of assembling amyloid fibrils to be estimated. The approach involves acquisition of real-time neutron scattering data during the initial stages of seeded growth, using contrast matched buffer to make the seeds effectively invisible to neutrons. As deuterated monomers add on to the seeds, the labelled growing ends give rise to scattering patterns that we model as cylinders whose increase in length with time gives an elongation rate. In addition, the absolute intensity of the signal can be used to determine the number of growing ends per unit volume, which in turn provides an estimate of seed length. The number of ends did not change significantly during elongation, demonstrating that any spontaneous

SUBMITTER: Eves BJ 

PROVIDER: S-EPMC8341957 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature