Cdc4 phospho-degrons allow differential regulation of Ame1^CENP-U protein stability across the cell cycle.

ABSTRACT: Kinetochores are multi-subunit protein assemblies that link chromosomes to microtubules of the mitotic and meiotic spindle. It is still poorly understood how efficient, centromere-dependent kinetochore assembly is accomplished from hundreds of individual protein building blocks in a cell cycle-dependent manner. Here, by combining comprehensive phosphorylation analysis of native Ctf19^CCAN subunits with biochemical and functional assays in the model system budding yeast, we demonstrate that Cdk1 phosphorylation activates phospho-degrons on the essential subunit Ame1^CENP-U, which are recognized by the E3 ubiquitin ligase complex SCF-Cdc4. Gradual phosphorylation of degron motifs culminates in M-phase and targets the protein for degradation. Binding of the Mtw1^Mis12<

SUBMITTER: Bohm M 

PROVIDER: S-EPMC8341979 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature