Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids.

ABSTRACT: Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with brain extracellular matrix significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augment

SUBMITTER: Cho AN 

PROVIDER: S-EPMC8342542 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature