A unique esophageal extracellular matrix proteome alters normal fibroblast function in severe eosinophilic esophagitis.

ABSTRACT:

Background

Eosinophilic esophagitis (EoE) is a chronic T_H2 disorder complicated by tissue fibrosis and loss of esophageal luminal patency. The fibrostenotic esophagus does not respond well to therapy, but profibrotic therapeutic targets are largely unclear.

Objective

Our aim was to utilize proteomics and primary cells as a novel approach to determine relevant profibrotic factors.

Methods

We utilized primary esophageal EoE and normal fibroblasts, their derivative extracellular matrixes (ECMs), an approach of fibroblast culture on autologous versus nonautologous ECM, and proteomics to elucidate EoE ECM proteins that dysregulate cellular function.

Results

We cultured esophageal fibroblasts from normal esophagi and esophagi from patients with severe EoE

SUBMITTER: Hsieh LY 

PROVIDER: S-EPMC8342625 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature