A single cell atlas of human cornea that defines its development, limbal progenitor cells and their interactions with the immune cells.

ABSTRACT:

Purpose

Single cell (sc) analyses of key embryonic, fetal and adult stages were performed to generate a comprehensive single cell atlas of all the corneal and adjacent conjunctival cell types from development to adulthood.

Methods

Four human adult and seventeen embryonic and fetal corneas from 10 to 21 post conception week (PCW) specimens were dissociated to single cells and subjected to scRNA- and/or ATAC-Seq using the 10x Genomics platform. These were embedded using Uniform Manifold Approximation and Projection (UMAP) and clustered using Seurat graph-based clustering. Cluster identification was performed based on marker gene expression, bioinformatic data mining and immunofluorescence (IF) analysis. RNA interference, IF, colony forming efficiency and clonal assays were per

SUBMITTER: Collin J 

PROVIDER: S-EPMC8343164 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature