Coating with flexible DNA network enhanced T-cell activation and tumor killing for adoptive cell therapy.

ABSTRACT: Adoptive cell therapy (ACT) is an emerging powerful cancer immunotherapy, which includes a complex process of genetic modification, stimulation and expansion. During these in vitro or ex vivo manipulation, sensitive cells are inescapability subjected to harmful external stimuli. Although a variety of cytoprotection strategies have been developed, their application on ACT remains challenging. Herein, a DNA network is constructed on cell surface by rolling circle amplification (RCA), and T cell-targeted trivalent tetrahedral DNA nanostructure is used as a rigid scaffold to achieve high-efficient and selective coating for T cells. The cytoprotective DNA network on T-cell surface makes them aggregate over time to form cell clusters, which exhibit more resistance to external stimu

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC8343197 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature