Development of an RNAi-based microalgal larvicide for the control of Aedes aegypti.

ABSTRACT:

Background

Mosquito-borne diseases affect over half of the human population globally. Multiple studies have shown that chemical insecticides are ineffective because of resistance. Therefore, environmentally safe mosquito population control tools need to be developed. Ribonucleic acid interference (RNAi) is a reverse genetic mechanism recently introduced as a new pest control tool. This technique represents a new class of biorational technology that could combat the increased global incidence of insecticide resistance. The technique has the potential of becoming a critical component of integrated vector control programs.

Methods

A 3-hydroxykynurenine transaminase (3-HKT) RNAi expression plasmid was constructed, generated and transformed into Chlamydomonas and Chlorella algae.

SUBMITTER: Fei X 

PROVIDER: S-EPMC8344188 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature