The role of 25-hydroxyvitamin-D3 and vitamin D receptor gene in human periodontal ligament fibroblasts as response to orthodontic compressive strain: an in vitro study.

ABSTRACT:

Background

This study aimed to investigate, if different physiological concentrations of vitamin D (25(OH)D₃) and single nucleotide polymorphisms in vitamin D receptor (VDR) gene have an impact on gene expression in human periodontal ligament (hPDL) fibroblasts induced by simulated orthodontic compressive strain.

Methods

A pool of hPDL fibroblasts was treated in absence or presence of 25(OH)D₃ in 3 different concentrations (10, 40 and 60 ng/ml). In order to evaluate the role of single nucleotide polymorphisms in the VDR gene, hPDL fibroblasts from 9 patients were used and treated in absence or presence of 40 ng/ml 25(OH)D₃. Each experiment was performed with and without simulated orthodontic compressive strain. Real-time PCR was used for gene

SUBMITTER: Kuchler EC 

PROVIDER: S-EPMC8344219 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature