Prediction of Protein Complex Structure Using Surface-Induced Dissociation and Cryo-Electron Microscopy.

ABSTRACT: A variety of techniques involving the use of mass spectrometry (MS) have been developed to obtain structural information on proteins and protein complexes. One example of these techniques, surface-induced dissociation (SID), has been used to study the oligomeric state and connectivity of protein complexes. Recently, we demonstrated that appearance energies (AE) could be extracted from SID experiments and that they correlate with structural features of specific protein-protein interfaces. While SID AE provides some structural information, the AE data alone are not sufficient to determine the structures of the complexes. For this reason, we sought to supplement the data with computational modeling, through protein-protein docking. In a previous study, we demonstrated that the scoring of stru

SUBMITTER: Seffernick JT 

PROVIDER: S-EPMC8344370 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature