Collagen Organization Does Not Influence T-Cell Distribution in Stroma of Human Pancreatic Cancer.

ABSTRACT: The dominant intrastromal T-cell infiltration in pancreatic cancer is mainly caused by the contact guidance through the excessive desmoplastic reaction and could represent one of the obstacles to an effective immune response in this tumor type. This study analyzed the collagen organization in normal and malignant pancreatic tissues as well as its influence on T-cell distribution in pancreatic cancer. Human pancreatic tissue was analyzed using immunofluorescence staining and multiphoton and SHG microscopy supported by multistep image processing. The influence of collagen alignment on activated T-cells was studied using 3D matrices and time-lapse microscopy. It was found that the stroma of malignant and normal pancreatic tissues was characterized by complex individual organization. T-cells w

SUBMITTER: Kamionka EM 

PROVIDER: S-EPMC8344977 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature