Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.

ABSTRACT: Keratins are the main identification markers of circulating tumor cells (CTCs); however, whether their deregulation is associated with the metastatic process is largely unknown. Previously we have shown by in silico analysis that keratin 16 (KRT16) mRNA upregulation might be associated with more aggressive cancer. Therefore, in this study, we investigated the biological role and the clinical relevance of K16 in metastatic breast cancer. By performing RT-qPCR, western blot, and immunocytochemistry, we investigated the expression patterns of K16 in metastatic breast cancer cell lines and evaluated the clinical relevance of K16 expression in CTCs of 20 metastatic breast cancer patients. High K16 protein expression was associated with an intermediate mesenchymal phenotype. Functional st

SUBMITTER: Elazezy M 

PROVIDER: S-EPMC8345379 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature