Ontology highlight
ABSTRACT:
SUBMITTER: Duysak T
PROVIDER: S-EPMC8346002 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Duysak Taner T Tran Thanh Tuyen TT Afzal Aqeel Rana AR Jung Che-Hun CH
International journal of molecular sciences 20210723 15
The cyclic AMP receptor protein (CRP) is one of the best-known transcription factors, regulating about 400 genes. The histone-like nucleoid structuring protein (H-NS) is one of the nucleoid-forming proteins and is responsible for DNA packaging and gene repression in prokaryotes. In this study, the binding of ppGpp to CRP and H-NS was determined by fluorescence spectroscopy. CRP from <i>Escherichia coli</i> exhibited intrinsic fluorescence at 341 nm when excited at 280 nm. The fluorescence intens ...[more]