Fasting Hormones Synergistically Induce Amino Acid Catabolism Genes to Promote Gluconeogenesis.

ABSTRACT:

Background & aims

Gluconeogenesis from amino acids (AAs) maintains glucose homeostasis during fasting. Although glucagon is known to regulate AA catabolism, the contribution of other hormones to it and the scope of transcriptional regulation dictating AA catabolism are unknown. We explored the role of the fasting hormones glucagon and glucocorticoids in transcriptional regulation of AA catabolism genes and AA-dependent gluconeogenesis.

Methods

We tested the RNA expression of AA catabolism genes and glucose production in primary mouse hepatocytes treated with fasting hormones (glucagon, corticosterone) and feeding hormones (insulin, fibroblast growth factor 19). We analyzed genomic data of chromatin accessibility and chromatin immunoprecipitation in mice and primary mouse hep

SUBMITTER: Korenfeld N 

PROVIDER: S-EPMC8346669 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature