Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state.

ABSTRACT: Polyhydroxyalkanoates (PHAs) are biodegradable polyesters that are intracellularly accumulated as distinct insoluble granules by various microorganisms. PHAs have attracted much attention as sustainable substitutes for petroleum-based plastics. However, the formation of PHA granules and their characteristics, such as localization, volume, weight, and density of granules, in an individual live bacterial cell are not well understood. Here, we report the results of three-dimensional (3D) quantitative label-free analysis of PHA granules in individual live bacterial cells through measuring the refractive index distributions by optical diffraction tomography (ODT). The formation and growth of PHA granules in the cells of Cupriavidus necator, the best-studied native PHA producer, and recom

SUBMITTER: Choi SY 

PROVIDER: S-EPMC8346791 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature