Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives.

ABSTRACT: The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin. Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available. Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies. Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia. We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5. A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous e

SUBMITTER: Bonaccorsi di Patti MC 

PROVIDER: S-EPMC8347646 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature