Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming.

ABSTRACT:

Objectives

Cell reprogramming has significant impacts on their potential application in regenerative medicine. Chromatin remodelling plays a very important role in cell reprogramming, but its underlying mechanism remains poorly understood.

Materials and methods

RNA-seq, quantitative RT-PCR and western blot analysis were applied to study the role of RNF20 and H2B ubiquitination during mouse somatic cell reprogramming. Chromatin structure and the recruitment of transcription factors were analysed by ChIP-seq, micrococcal nuclease sensitivity assays and immunofluorescence staining.

Results

We show that RNF20 is highly expressed at the early stage of reprogramming along with the accumulation of H2B ubiquitination at the same stage, and Rnf20 knockout results in the failur

SUBMITTER: Wang L 

PROVIDER: S-EPMC8349662 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature