Mapping of m⁶A and Its Regulatory Targets in Prostate Cancer Reveals a METTL3-Low Induction of Therapy Resistance.

ABSTRACT: Recent evidence has highlighted the role of N ⁶-methyladenosine (m⁶A) in the regulation of mRNA expression, stability, and translation, supporting a potential role for posttranscriptional regulation mediated by m⁶A in cancer. Here, we explore prostate cancer as an exemplar and demonstrate that low levels of N ⁶-adenosine-methyltransferase (METTL3) is associated with advanced metastatic disease. To investigate this relationship, we generated the first prostate m⁶A maps, and further examined how METTL3 regulates expression at the level of transcription, translation, and protein. Significantly, transcripts encoding extracellular matrix proteins are consistently upregulated with METTL3 knockdown. We also examine

SUBMITTER: Cotter KA 

PROVIDER: S-EPMC8349875 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature