H₂O₂-mediated autophagy during ethanol metabolism.

ABSTRACT:

Background

Alcoholic liver disease (ALD) is the most common liver disease worldwide and its underlying molecular mechanisms are still poorly understood. Moreover, conflicting data have been reported on potentially protective autophagy, the exact role of ethanol-metabolizing enzymes and ROS.

Methods

Expression of LC3B, CYP2E1, and NOX4 was studied in a mouse model of acute ethanol exposure by immunoblotting and immunohistochemistry. Autophagy was further studied in primary mouse hepatocytes and huh7 cells in response to ethanol and its major intermediator acetaldehyde. Experiments were carried out in cells overexpressing CYP2E1 and knock down of NOX4 using siRNA. The response to external H₂O₂ was studied by using the GOX/CAT system. Autophagic flux was m

SUBMITTER: Chen C 

PROVIDER: S-EPMC8350071 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature