Activated mast cells in skeletal muscle can be a potential mediator for cancer-associated cachexia.

ABSTRACT:

Background

Eighty per cent of United States advanced cancer patients faces a worsened prognosis due to cancer-associated cachexia. Inflammation is one driver of muscle atrophy in cachexia, and skeletal muscle-resident immune cells could be a source of inflammation. This study explores the efficacy of cancer activated skeletal muscle-resident mast cells as a biomarker and mediator of cachexia.

Methods

Individual gene markers for immune cells were assessed in a publicly available colon carcinoma cohort of normal (n = 3), moderate cachexia (n = 3), and severe cachexia (n = 4) mice. Lewis lung carcinoma (LL/2) cells induced cachexia in C57BL/6 mice, and a combination of toluidine blue staining, immunofluorescence, quantitative polymerase chain reaction, and western blots measure

SUBMITTER: Widner DB 

PROVIDER: S-EPMC8350201 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature