Single-Cell Proteomics Reveals the Defined Heterogeneity of Resident Macrophages in White Adipose Tissue.

ABSTRACT: Adipose tissue macrophages (ATMs) regulate homeostasis and contribute to the metabolically harmful chronic inflammation in obese individuals. While evident heterogeneity of resident ATMs has been described previously, their phenotype, developmental origin, and functionality remain inconsistent. We analyzed white adipose tissue (WAT) during homeostasis and diet interventions using comprehensive and unbiased single-cell mass cytometry and genetic lineage tracking models. We now provide a uniform definition of individual subsets of resident ATMs. We show that in lean mice, WAT co-harbors eight kinetically evolving CD206⁺ macrophage subpopulations (defined by TIM4, CD163, and MHC II) and two CD206^- macrophage subpopulations. TIM4^-CD163⁺, TIM4^-</

SUBMITTER: Felix I 

PROVIDER: S-EPMC8350344 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature