Redox index of Cys-thiol residues of serum apolipoprotein E and its diagnostic potential.

ABSTRACT: The redox modulation of Cys-thiol participates in various pathophysiological processes. We explored the proper index for estimating the redox status of Cys-thiol of serum apolipoprotein E (apoE), named "redox-IDX-apoE," which is necessary to understand the redox biology of age-related diseases. The fractions of the reduced form (red-), reversible oxidized form (roxi-), and irreversibly oxidized form (oxi-) apoE in serum, obtained from the patients with no apparent disease (controls, n=192) and with atherosclerosis and type 2 diabetes (patients, n=16), were measured by a band-shift assay using a maleimide compound. Redox-IDX-apoE candidates were determined by calculating the values of these fractions and the total apoE concentration. Cys number of apoE significantly increased for the ratio

SUBMITTER: Yamauchi K 

PROVIDER: S-EPMC8350432 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature