Selection of high-producing clones by a relative titer predictive model using image analysis.

ABSTRACT:

Background

The commercial success of monoclonal antibodies (Mabs) has made biological therapeutics attractive to pharmaceutical companies. The priority of biopharmaceutical companies is to acquire and develop cell lines that enable them to manufacture biologics quickly, consistently, and economically. Clone selection is a critical process for cell line development. However, the traditional clone selection process requires the evaluation of large numbers of clones using cell growth rate, cell densities and titer, product quality, and so on.

Methods

To improve efficiency of the clone selection strategies, we developed a relative titer (RT) prediction model by the quantitative information extracted from microscope images during the cell line development process. The performance

SUBMITTER: Tao W 

PROVIDER: S-EPMC8350677 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature