Fragmentation patterns and personalized sequencing of cell-free DNA in urine and plasma of glioma patients.

ABSTRACT: Glioma-derived cell-free DNA (cfDNA) is challenging to detect using liquid biopsy because quantities in body fluids are low. We determined the glioma-derived DNA fraction in cerebrospinal fluid (CSF), plasma, and urine samples from patients using sequencing of personalized capture panels guided by analysis of matched tumor biopsies. By sequencing cfDNA across thousands of mutations, identified individually in each patient's tumor, we detected tumor-derived DNA in the majority of CSF (7/8), plasma (10/12), and urine samples (10/16), with a median tumor fraction of 6.4 × 10^-3 , 3.1 × 10^-5 , and 4.7 × 10^-5 , respectively. We identified a shift in the size distribution of tumor-derived cfDNA fragments in these body fluids. We further analyzed cfDNA fragment siz

SUBMITTER: Mouliere F 

PROVIDER: S-EPMC8350897 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature