Screening and evaluation of the strong endogenous promoters in Pichia pastoris.

ABSTRACT:

Background

Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds. However, basic research on P. pastoris lags far behind Saccharomyces cerevisiae, resulting in a lack of available biological elements. Especially, fewer strong endogenous promoter elements available for foreign protein expression or construction of biosynthetic pathways were carefully evaluated in P. pastoris. Thus, it will be necessary to identify more available endogenous promoters from P. pastoris.

Results

Based on RNA-seq and LacZ reporter system, eight strong endogenous promoters contributing to higher transcriptional

SUBMITTER: Dou W 

PROVIDER: S-EPMC8351359 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature