Using a new HSPC senescence model in vitro to explore the mechanism of cellular memory in aging HSPCs.

ABSTRACT:

Background

Age-associated changes attenuate human blood system functionality through the aging of hematopoietic stem and progenitor cells (HSPCs), manifested in human populations an increase in myeloproliferative disease and even leukemia; therefore, study on HSPC senescence bears great significance to treat hematopoietic-associated disease. Furthermore, the mechanism of HSPC aging is lacking, especially the cellular memory mechanism. Here, we not only reported a new HSPC senescence model in vitro, but also propose and verify the cellular memory mechanism of HSPC aging of the Polycomb/Trithorax system.

Methods

HSPCs (Lin^-c-kit⁺ cells) were isolated and purified by magnetic cell sorting (MACS). The proportions and cell cycle distribution of cells were de

SUBMITTER: Dong Y 

PROVIDER: S-EPMC8351417 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature