Identification of miRNA-target gene regulatory networks in liver fibrosis based on bioinformatics analysis.

ABSTRACT:

Background

Liver cirrhosis is one of the leading causes of death worldwide. MicroRNAs (miRNAs) can regulate liver fibrosis, but the underlying mechanisms are not fully understood, and the interactions between miRNAs and mRNAs are not clearly elucidated.

Methods

miRNA and mRNA expression arrays of cirrhotic samples and control samples were acquired from the Gene Expression Omnibus database. miRNA-mRNA integrated analysis, functional enrichment analysis and protein-protein interaction (PPI) network construction were performed to identify differentially expressed miRNAs (DEMs) and mRNAs (DEGs), miRNA-mRNA interaction networks, enriched pathways and hub genes. Finally, the results were validated with in vitro cell models.

Results

By bioinformatics analysis, we iden

SUBMITTER: Tai Y 

PROVIDER: S-EPMC8351572 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature