Autophagy inhibition reinforces stemness together with exit from dormancy of polydisperse glioblastoma stem cells.

ABSTRACT: Therapeutic resistance and infiltrative capacities justify the aggressiveness of glioblastoma. This is due to cellular heterogeneity, especially the presence of stemness-related cells, i.e. Cancer Stem Cells (CSC). Previous studies focused on autophagy and its role in CSCs maintenance; these studies gave conflicting results as they reported either sustaining or disruptive effects. In the present work, we silenced two autophagy related genes -either Beclin1 or ATG5- by shRNA and we explored the ensuing consequences on CSCs markers' expression and functionalities. Our results showed that the down regulation of autophagy led to enhancement in expression of CSCs markers, while proliferation and clonogenicity were boosted. Temozolomide (TMZ) treatment failed to induce apoptotic death in shBecli

SUBMITTER: Brunel A 

PROVIDER: S-EPMC8351723 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature