Noninvasive assessment of radiation-induced renal injury in mice.

ABSTRACT:

Purpose

The kidney is a radiosensitive late-responding normal tissue. Injury is characterized by radiation nephropathy and decline of glomerular filtration rate (GFR). The current study aimed to compare two rapid and cost-effective methodologies of assessing GFR against more conventional biomarker measurements.

Methods

C57BL/6 mice were treated with bilateral focal X-irradiation (1x14Gy or 5x6Gy). Functional measurements of kidney injury were assessed 20 weeks post-treatment. GFR was estimated using a transcutaneous measurement of fluorescein-isothiocyanate conjugated (FITC)-sinistrin renal excretion and also dynamic contrast-enhanced CT imaging with a contrast agent (ISOVUE-300 Iopamidol).

Results

Hematoxylin and eosin (H&E) and Periodic acid-Schiff staining identifi

SUBMITTER: Ahmad A 

PROVIDER: S-EPMC8352084 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature