Mesenteric Lymph Node Transplantation in Mice to Study Immune Responses of the Gastrointestinal Tract.

ABSTRACT: Mesenteric lymph nodes (mLNs) are sentinel sites of enteral immunosurveillance and immune homeostasis. Immune cells from the gastrointestinal tract (GIT) are constantly recruited to the mLNs in steady-state and under inflammatory conditions resulting in the induction of tolerance and immune cells activation, respectively. Surgical dissection and transplantation of lymph nodes (LN) is a technique that has supported seminal work to study LN function and is useful to investigate resident stromal and endothelial cell biology and their cellular interactions in experimental disease models. Here, we provide a detailed protocol of syngeneic mLN transplantation and report assays to analyze effective mLN engraftment in congenic recipients. Transplanted mLNs allow to study T cell activation and proli

SUBMITTER: Shaikh H 

PROVIDER: S-EPMC8352558 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature