Maternally inherited piRNAs direct transient heterochromatin formation at active transposons during early <i>Drosophila</i> embryogenesis.

ABSTRACT: The PIWI-interacting RNA (piRNA) pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. In Drosophila, piRNAs are intergenerationally inherited through the maternal lineage, and this has demonstrated importance in the specification of piRNA source loci and in silencing of I- and P-elements in the germ cells of daughters. Maternally inherited Piwi protein enters somatic nuclei in early embryos prior to zygotic genome activation and persists therein for roughly half of the time required to complete embryonic development. To investigate the role of the piRNA pathway in the embryonic soma, we created a conditionally unstable Piwi protein. This enabled maternally deposited Piwi to be cleared from newly laid embryos w

SUBMITTER: Fabry MH 

PROVIDER: S-EPMC8352587 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature