Highly localized intracellular Ca²⁺ signals promote optimal salivary gland fluid secretion.

ABSTRACT: Salivary fluid secretion involves an intricate choreography of membrane transporters to result in the trans-epithelial movement of NaCl and water into the acinus lumen. Current models are largely based on experimental observations in enzymatically isolated cells where the Ca²⁺ signal invariably propagates globally and thus appears ideally suited to activate spatially separated Cl and K channels, present on the apical and basolateral plasma membrane, respectively. We monitored Ca²⁺ signals and salivary secretion in live mice expressing GCamp6F, following stimulation of the nerves innervating the submandibular gland. Consistent with in vitro studies, Ca²⁺ signals were initiated in the apical endoplasmic reticulum. In marked contrast to in vitro data, highly l

SUBMITTER: Takano T 

PROVIDER: S-EPMC8352588 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature