Comparative analysis of freshwater phytoplankton communities in two lakes of Burabay National Park using morphological and molecular approaches.

ABSTRACT: We analyzed phytoplankton assemblages' variations in oligo-mesotrophic Shchuchie and Burabay lakes using traditional morphological and next-generation sequencing (NGS) approaches. The total phytoplankton biodiversity and abundance estimated by both microscopy and NGS were significantly higher in Lake Burabay than in Lake Shchuchie. NGS of 16S and 18S rRNA amplicons adequately identify phytoplankton taxa only on the genera level, while species composition obtained by microscopic examination was significantly larger. The limitations of NGS analysis could be related to insufficient coverage of freshwater lakes phytoplankton by existing databases, short algal sequences available from current instrumentation, and high homology of chloroplast genes in eukaryotic cells. However, utilization of NG

SUBMITTER: Malashenkov DV 

PROVIDER: S-EPMC8352915 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature