Reprogramming enriches for somatic cell clones with small-scale mutations in cancer-associated genes.

ABSTRACT: Cellular therapies based on induced pluripotent stem cells (iPSCs) come out of age and an increasing number of clinical trials applying iPSC-based transplants are ongoing or in preparation. Recent studies, however, demonstrated a high number of small-scale mutations in iPSCs. Although the mutational load in iPSCs seems to be largely derived from their parental cells, it is still unknown whether reprogramming may enrich for individual mutations that could lead to loss of functionality and tumor formation from iPSC derivatives. 30 hiPSC lines were analyzed by whole exome sequencing. High accuracy amplicon sequencing showed that all analyzed small-scale variants pre-existed in their parental cells and that individual mutations present in small subpopulations of parental cells become enriched

SUBMITTER: Kosanke M 

PROVIDER: S-EPMC8353200 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature