Quantitative analysis of m6A RNA modification by LC-MS.
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ABSTRACT:
SUBMITTER: Mathur L
PROVIDER: S-EPMC8353476 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
Quantitative analysis of m<sup>6</sup>A RNA modification by LC-MS.
Mathur Lavina L Jung Sunhee S Jang Cholsoon C Lee Gina G
STAR protocols 20210806 3
<i>N</i><sup>6</sup>-adenosine methylation (m<sup>6</sup>A) of messenger RNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m<sup>6</sup>A levels is thus critical to understand its dynamic changes in various biological settings. Here, we provide a protocol to quantitate the levels of adenosine and m<sup>6</sup>A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatography mass spec ...[more]
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